Tag: biotechnology

Questions Related to biotechnology

Genetic engineering is possible because

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  1. The phenomenon of transduction in bacteria is well understood.

  2. We can see DNA by electron microscope.

  3. We can cut DNA at specific sites by endonucleases like DNAase I.

  4. Restriction endonucleases purified from bacteria can be used in vitro.

Show answer
Correct Option: D
Explanation:

Genetic engineering is possible because all organisms are based on the same genetic code. DNases which act on specific positions or sequences on the DNA are called as restriction endonucleases. The sequences which are recognised by the restriction endonucleases or restriction enzymes (RE) are called as recognition sequences restriction sites. These sequences are palindromic sequences. Different restriction enzymes present in different bacteria can recognize different or same restriction sites but they will cut at two different points within the restriction site. No two restriction enzymes from a single bacterium will cut at the same restriction site. 

Thus, the correct answer is option D.

Which of the following is not a tool of genetic engineering?

botany biotechnology: general introduction microbiology: organisms in industry biotechnology commercial applications of biotechnology

  1. Cloning vector

  2. Restriction enzyme

  3. Foreign DNA

  4. GMO

Show answer
Correct Option: D
Explanation:

Genetic engineering is the process of replicating a specific set of genes from a strand of DNA. It is the process of using gene cloning and other genetic manipulation tools to achieve isolate specific genes. 

Tools for genetic engineering:
  • PCR
  • Restriction enzymes
  • Gel electrophoresis
  • DNA Ligase
  • Plasmids 
  • Transformation 
  • Transgenic organisms 
  • So, the correct option is 'Option D'.

An organic compound has a triple bond and double bond. It can be tested by :

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  1. Bromine water

  2. Beyer's reagents

  3. Fehling's solution

  4. Ammonical $AgNO _3$

Show answer
Correct Option: A
Explanation:
The bromine test is a qualitative test for the presence of unsaturation (carbon-to-carbon double or triple bonds) and phenols. An unknown sample is treated with a small amount of elemental bromine in an organic solvent, being as dichloromethane or carbon tetrachloride. Presence of unsaturation and/or phenol in the sample is shown by disappearance of the deep brown coloration of bromine when it has reacted with the unknown sample. 
So, the correct option is 'Bromine water'.

What is common between NAD and FAD?

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  1. Both are coenzymes

  2. Both are derived from proteins

  3. Both acts as oxygen carriers

  4. All of the above

Show answer
Correct Option: A
Explanation:

Nicotinamide Adenine Dinucleotide (NAD) and Flavin Adenine Dinucleotide (FAD) are coenzymes associated with reversible oxidation and decrease reactions. Then, these lessened coenzymes can give these electrons to some other biochemical response ordinarily engaged with a procedure that is anabolic (like the union of ATP).

So, the correct option is 'Both are coenzymes'.

Match and choose the true option.

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  1. $NaCl$ - Inorganic micromolecule

  2. ${H} _{2}O$ - Organic micromolecule

  3. Starch - Organic micromolecule

  4. Glucose - Inorganic macromolecule

Show answer
Correct Option: A
Explanation:
Organic molecules are made of chains of carbon atoms. Inorganic molecules are minerals like salts NaCl which held together by an ionic bond.
Water doesn't contain any carbon atom in its molecule.  So water is an inorganic compound. 
So the correct answer is A. 
Biomolecules are
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  1. inorganic materials

  2. organic materials

  3. all the carbon compounds obtained from living tissues

  4. only DNA and RNA

Show answer
Correct Option: C
Explanation:

Biomolecules are basically all the carbon compounds obtained from the living tissue. All the major components that make up a living being that is the carbohydrate, proteins, lipids contain carbon in one form or the other.

Carbon is the structural as well as the functional unit of the basic framework of the cell.

So, the correct option is 'all the carbon compounds obtained from living tissues'

Cellular micromolecules are

biomacromolecules biotechnology cell biology and biotechnology biomolecules biology

  1. Amino acids, Water, Minerals and Sugars

  2. Glycogen, Amino acids, Minerals and Nucleotides

  3. Water, Minerals, Nucleic acids, Amino acids and Nucleotides

  4. Sugars, Water, Minerals, Proteins and Nucleotides

Show answer
Correct Option: A
Explanation:

The cellulose micromolecules are small in size and have low molecular weights which is less than 1000 daltons. They have simple molecular structure and often high solubility in the intracellular fluid. These include inorganic compounds such as water, mineral salts, and gases as well as organic compounds such as sugars, lipids, amino acids and nucleotides. The small molecules are the substrates and products of metabolism in the cell. The micromolecules act as building blocks for the macromolecules. 

Thus, the correct answer is option A. 

All organic substances possess

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  1. Carbon, Hydrogen and Oxygen

  2. Carbon, Oxygen and Nitrogen

  3. Carbon and Hydrogen

  4. Carbon, Hydrogen, Oxygen and Nitrogen

Show answer
Correct Option: C
Explanation:
The organic compound contain the element carbon. The carbon atoms are bonded to form a chain or ring which serves as a skeleton to organic molecule. The most of their carbon atoms are also bonded to one or more hydrogen atoms. The organic compounds are chiefly formed by living organisms. They have large molecules with complex structure and high molecular weights. They include carbohydrates, lipids, proteins, nucleotides and nucleic acids
So, the correct answer is 'Carbon and Hydrogen'

Which of the following is nucleo-proteinaceous?

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  1. Chromosomes, viruses and ribosomes

  2. Centriole, viruses and lysosomes

  3. VIruses, chromosomes and vacuole

  4. Nucleus, GB, DNA

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Correct Option: A

About 98 percentage of the mass of every living organism is composed of just six elements including carbon, hydrogen, nitrogen, oxygen and

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  1. Sulphur and Magnesium

  2. Magnesium and Phosphorous

  3. Calcium and Phosphorous

  4. Phosphorus and Sulphur

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Correct Option: C
Explanation:
  • Body needs phosphorus to build and repair bones and teeth.
  • It also helps nerves to function.
  • Calcium is important on blood clotting, muscle contraction, and strengthening bones.