Tag: molecular biology

cDNA or complementary DNA is the DNA synthesized from a single-stranded RNA template in a reaction catalysed by reverse transcriptase enzyme. This cDNA is naturally produced by retroviruses where RNA acts as the genetic material. It is also used to clone eukaryotic genes in prokaryotes.

Most DNA molecules consist of two biopolymer strands coiled around each other to form a double helix. The two DNA strands are known as polynucleotides since, they are composed of simpler units called as nucleotides. Each nucleotide is composed of a nitrogen-containing nucleobase either guanine (G), adenine (A), thymine (T), or cytosine (C) as well as a monosaccharide sugar called as deoxyribose and a phosphate group. The nucleotides are joined to one another in a chain by covalent bonds between the sugar of one nucleotide and the phosphate of the next, resulting in an alternating sugar-phosphate backbone. According to base pairing rules (A with T and C with G), hydrogen bonds bind the nitrogenous bases of the two separate polynucleotide strands to make double-stranded DNA. So, nuclotides are building blocks of DNA and not glucose or aminoacid. Thus, the correct answer is option C.

0 x 174 Bacteriophage : In this bacteriophage, DNA is rounded, closed and bangle shaped, the duplication pattern is different type in this bacteriophage.

All the bio-molecules are important for life but as per the question the nucleic acids stand out, because they only have genetic information coded in them and can pass on this information to next generation of cells. So, nucleic acid plays a significant role  in the basis of life. 

• In DNA transcription, the coding strand is the DNA strand whose base sequence corresponds to the base sequence of the RNA transcript produced (although with thymine replaced by uracil).
•  It is this strand which contains codons, while the non-coding strand contains anticodons.
•  Hence, UAG is triplet nitrogen base on m RNA, TAG will be the corresponding triplet on the coding strand. 
•  So, the correct answer is 'TAG'.

In eukaryotes DNA is tightly bound to histones which form a DNA protein particles called nucleosome. Each nucleosome contains 2 copies of each $H _{2A},H _{2B}.H _{3}$ and $H _{4}$ histone protein bounded on 146 BP of DNA.Each nucleosome bead is linked to next nucleosome bead by linker DNA.

The transforming principle in Griffith's experiment is DNA. Griffith conducted an experiment in 1928. In this experiment he chose two strains of Stephylococcus pneumoniae bacteria. One of them was a smooth strain which was virulent and the other one was a rough strain which was non-virulent. The smooth strains caused pneumonia when they were injected to the mice, and the rough strains did not cause pneumonia in mice. Griffith heat killed the smooth strain bacteria and injected them into mice and he observed that pneumonia was not caused in them. He mixed the heat killed smooth strain and rough strain and injected the mixture into mice and this mixture caused pneumonia in mice. When he tested the blood of the mice, he saw both rough and smooth strains of bacteria. This implied that some factor from the dead smooth bacterial strain had taken entry into the rough strains and converted them into smooth strains. He called this factor as Transforming principle.