Tag: botany

Questions Related to botany

In retroviruses, RNA dependent DNA polymerase synthesizes

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. DNA

  2. RNA

  3. RNA-DNA

  4. None of the above

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Correct Option: C

Blood stain component to be used for DNA profiling technique is

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Serum

  2. Leucocytes

  3. Platelets

  4. Erythrocytes

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Correct Option: B

Read the following four statements (A-D) about certain mistakes in two of them:

(A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactalbumin enriched.
(B) Restriction enzymes are used in isolation of DNA from other macro-molecules.
(C) Downstream processing is one of the steps of r-DNA technology.
(D) Disarmed pathogen vectors are also used in transfer of r-DNA into the host.

Which are the two statements having mistakes?

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Statements (A) and (B)

  2. Statements (B) and (C)

  3. Statements (C) and (D)

  4. Statements (A) and (C)

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Correct Option: A
Explanation:

(A) Rosie was the first transgenic cow and its milk was rich in human alpha-lactalbumin protein.

(B) Restriction enzymes are used in the isolation of the specific segment of DNA from the DNA of the organism because these enzymes are capable of cutting the DNA at the specific location.
(C) Downstream processing is one of the steps in the r-DNA technology. It involves the extraction and purification of the desired product.
(D) Disarmed pathogens like Agrobacterium tumifaciens, phage viruses are also used in the transfer of r-DNA into the host.
Hence, the correct answer is '(A) and (B)'

Elution means

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Separation of DNA fragments on agarose gel

  2. Cutting and extraction of DNA bands from the agarose gel

  3. Making the DNA bands visible under UV radiation

  4. Isolation of alien DNA from the choice organism

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Correct Option: B

A dicotyledonous plant forms crown gall when

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Agrobacterium tumefaciens comes in contact with the plant

  2. Agrobacterium rhizogenes comes in contact with the plant

  3. A specific part of DNA from the Ti plasmid gets integrated with the plant chromosome

  4. A specific part of DNA from the Ri plasmid gets integrated with the plant chromosome

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Correct Option: C

Find the correct statement with respect to function or properties of RNA polymerase.

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Bacteria have $3$ different types of RNA polymerase

  2. RNA polymerase binds to promoter and initiate transcription

  3. It uses nucleoside monophosphate as substrate

  4. RNA polymerase polymerizes in a template independent manner

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Correct Option: A

Consider the following steps during recombinant DNA technology.
a. Breaking of donor DNA using restriction enzyme
b. Culture of cloned bacteria containing the fragment of donor DNA
c. Isolation of suitable plasmid
d. Insertion of recombinant DNA through cloning
e. Joining of donor DNA with a suitable plasmid using ligase
The correct sequence of these is?

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. $1, 3, 2, 4, 5$

  2. $3, 2, 1, 5, 4$

  3. $1, 3, 5, 4, 2$

  4. $5, 1, 3, 4, 2$

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Correct Option: A

When E coli with DNA $^{15}N ^{15}N $ is allowed to grow in a medium containing $^{14}N$ the number of DNA strands with $^{15}N$ after five generations is: 

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. 1

  2. 2

  3. 8

  4. 16

  5. 30

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Correct Option: A

Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Reverse transcriptase-production of.cDNA from mRNA

  2. DNA polymerase-used in polymerase chain reaction to amplify section of DNA

  3. DNA ligase-enzyme that cuts DNA, creating the sticky ends of restriction fragments

  4. Restriction enzyme-production of RFLPs

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Correct Option: C
Explanation:

Restriction enzymes cut DNA creating sticky ends of restriction fragments. While DNA ligase joins these sticky ends to form recombinant DNA. 

So, the correct option is 'DNA ligase-enzyme that cuts DNA, creating the sticky ends of restriction fragments'. 

After insertion of DNA segment within the sequence of Z-gene of bacteria, they are grown on chromogenic substrate. After the growth of bacterial colonies, they are identified as
i) Recombinants if colonies are blue- coloured.
ii) Recombinants if colonies are white coloured.
iii) Non-recombinants if colonies are blue- coloured.
iv) Non-recombinants if colonies are white coloured.

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. iii, iv

  2. i, iv

  3. i, ii

  4. ii, iii

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Correct Option: D