Tag: botany

Questions Related to botany

Which cannot be used for direct gene transfer 

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Biolistics (gene gun)

  2. Microinjection

  3. Electroporation

  4. Agrobacterium tumefacians

Show answer
Correct Option: D
Explanation:
  • Biolistics (gene gun), Microinjection and Electroporation are direct methods of gene transfer.
  • Agrobacterium tumefaciens is a gram-negative soil bacterium, which can cause crown gall tumors at wound sites of infected dicotyledonous plants.
  • Agrobacterium tumefaciens acts as a vector for the transfer of genetic material hence it cannot be used for direct gene transfer.
  • Hence Agrobacterium tumefaciens cannot be used for direct gene transfer.
  • So, the correct answer is 'Agrobacterium tumefaciens'.

Which of these is used as vector in gene therapy for SCID?

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Arbovirus

  2. Rotavirus

  3. Enterovirus

  4. Parvovirus

  5. Retrovirus

Show answer
Correct Option: E
Explanation:

In gene therapy trials, the retrovirus is used as a vector to treat X-linked severe combined immunodeficiency (X-SCID) represent the most successful application of gene therapy. The retrovirus genetic material is in the form of RNA molecules, and their hosts genetic material is in the form of DNA. When a retrovirus infects a host cell, it will introduce its RNA together with some enzymes, namely reverse transcriptase and integrase, into the cell. This RNA molecule from the retrovirus must produce a DNA copy from its RNA molecule before it can be integrated into the genetic material of the host cell. The process of producing a DNA copy from an RNA molecule is termed reverse transcription.

Transfer of DNA bands from agarose gel to nitrocellulose or nylon membrane is

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Southern transfer

  2. Western transfer

  3. Northern transfer

  4. Eastern transfer

  5. Gene transfer

Show answer
Correct Option: A

Gene gun can introduce genes into cells with the help of

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Plasmids

  2. Cosmids

  3. Microscopic pellets

  4. Phagemids

Show answer
Correct Option: C
Explanation:

In the gene gun method, microscopic pellets of gold or tungsten are coated with the transgene fragments and shot into the plant cells or tissues at high velocity. 

So the correct option is C.

The technique in which foreign DNA is precipitated over surface of metal particles for passing into target cells is __________.

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Microinjection

  2. Electroporation

  3. Particle gun

  4. Chemical mediated gene transfer

Show answer
Correct Option: C

Microparticles for coating with DNA to be bombarded with gene gun are made of

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Silver or platinum

  2. Platinum or zinc

  3. Silicon or platinum

  4. Gold or tungsten

Show answer
Correct Option: D

Biolistic gun is suitable for

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Transformation of plant cells

  2. Disarming pathogen vectors

  3. DNA finger printing

  4. Constructing recombinant DNA

Show answer
Correct Option: A

Agrobacterium tumefaciens is most widely used for gene transfer because of/ as 

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. It causes crown gall tumours

  2. Its ability to insert Ti plasmid into nuclear genome

  3. It can grow anywhere

  4. It has the ability to kill pathogenic bacteria

Show answer
Correct Option: B

Which one of the following is the method of gene transfer based on the use of short electrical impulses of high field strength?

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Ultrasoniation

  2. Electroporation

  3. Microprojectile

  4. Macro injection

Show answer
Correct Option: A

The most commonly used method of choice for gene transfer using immature embryos as explants is ______________.

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Microprojectile

  2. Electroporation

  3. Liposome medfeted

  4. Chemically stimulated

Show answer
Correct Option: A