Tag: gene cloning procedure

The denaturation stage of PCR takes about 1–2 minutes. The thermocycler then lowers the temperature to about 50° to 60°C, which allows the short, oligonucleotide primers to anneal to their complementary sequences on the single-stranded DNA molecules. The annealing stage of PCR lasts about 30 seconds. Hence, the whole cycle of gene amplification requires 30 minutes.

Polymerase chain reaction (PCR) is a technique that exponentially amplifies a single copy of a specific DNA segment. It generates thousands of copies of that specific DNA segment. After completion of one cycle, 2 copies are produced from a single DNA segment. After completion of the second cycle, 2$^2$ = 4 copies are produced. Similarly, after n$^{th}$ cycle, 2$^2$ copies are produced, where n is the number of cycles. Hence, after completion of 6 cycle, 2$^6$ = 64 copies will be produced. 

Polymerase Chain Reaction (PCR) is widely used the method in genetic engineering to prepare the multiple copies of desired DNA. It includes steps of denaturation, annealing extension and last amplification. As DNA is the polymer of four DNA bases present in the base pair and polymerase chain reaction is done in the presence of DNA polymerase enzyme of the DNA that re-studies by the researchers which is said to be DNA library so it is not required in the reaction.