Tag: genetically engineered insulin

Multiple Choice Question

Globular protein of ~ 6kDaconsisting of 51 amino acids arranged in two polypeptide chains held by disulphide bridge is

Genetic engineering has helped in production of

Which one of correct?

Maturation of proinsulin into insulin takes place after

Recombinant DNA bearing ampicillin resistance gene is passed in E. coli. The latter are spread on agar plates containing ampicillin. Then

Escherichia coli having plasmids for production of human insulin is cultured in medium containing __________.

Some of the steps involved in the production of humulin are given below. Choose the correct sequence
(i)   Synthesis of insulin gene artificially
(ii)  Culturing recombinant E. coli in bioreactors
(iii) Purification of humulin
(iv) Insertion of human insulin gene into a plasmid
(v)  Introduction of recombinant plasmid into E.coli
(vi) Extraction of a recombinant gene product from E. coli

In the technique of recombinant insulin production, the genes for $\alpha$- and $\beta$ - polypeptides were inserted into the plasmid by the side of __________.

Human insulin is being commercially produced from transgenic species of _____________.