Tag: hgp and dna fingerprinting

Questions Related to hgp and dna fingerprinting

DNA profiling can be carried out using

zoology genetics of the future hgp and dna fingerprinting dna fingerprinting human genome project and dna fingerprinting

  1. Blood

  2. Saliva

  3. Both A and B

  4. None of the above

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Correct Option: C
Explanation:

DNA profiling can be carried out using a sample such as blood, saliva, semen or other appropriate fluid or tissue from personal items (e.g., a toothbrush, razor) or from stored samples (e.g., banked sperm or biopsy tissue). Samples obtained from blood relatives (related by birth, not marriage) can provide an indication of an individual's profile, as could human remains that had been previously profiled.

Which one of the following scientists developed the process of DNA fingerprinting?

zoology genetics of the future hgp and dna fingerprinting dna fingerprinting human genome project and dna fingerprinting

  1. Kary B. Mullis

  2. T. H. Morgan

  3. H. O. Smith

  4. Alec Jeffreys

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Correct Option: D
Explanation:

Sir Alec Jeffreys, professor of genetics is the pioneer of DNA profiling. DNA profiling (also called as DNA fingerprinting) is a forensic technique used to identify individuals by characteristics of their DNA.

The commonly used DNA fingerprinting technique in forensic science study is

zoology genetics of the future hgp and dna fingerprinting dna fingerprinting human genome project and dna fingerprinting

  1. Southern blotting

  2. Northern blotting

  3. Eastern blotting

  4. Western blotting

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Correct Option: A
Explanation:

Southern blotting and hybridization with probes can be used to locate a few specific fragments in a large pool of DNA. Southern blotting (named after Edwin M. Southern) is one technique used to transfer the denatured, single-stranded fragments from a gel to a thin solid medium.

DNA fingerprinting is useful for the study of

zoology genetics of the future hgp and dna fingerprinting dna fingerprinting human genome project and dna fingerprinting

  1. Forensic science

  2. Identity

  3. Relationship

  4. All of the above

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Correct Option: D
Explanation:

DNA fingerprinting is a method used in the identification of the genetic information (DNA) in a person's cell. It is a forensic technique used to identify an individual by the characteristics of the DNA. It is also useful to identify the identity and relation of an individual with others.

The technique used to increase the amount of DNA available for analysis is
I. Cloning a plasmid
II. Gel electrophoresis
III. PCR

zoology genetics of the future hgp and dna fingerprinting dna fingerprinting human genome project and dna fingerprinting

  1. II only

  2. I and II

  3. I and III

  4. II and III

  5. I, II, and III

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Correct Option: C
Explanation:
The polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy of DNA to millions of copies of a particular DNA sequence. 
Molecular cloning is a method used to assemble recombinant DNA molecules and to direct their replication within host organisms to increase the number of recombinant DNA molecule. Here, the DNA can be cloned in a plasmid (extracellular DNA).
Thus, the correct answer is option C.


Which of the following is not required for any of the techniques of DNA finger printing available at present

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  1. DNA-DNA hybridisation

  2. Polymerase chain reaction

  3. Zinc finger analysis

  4. Restriction enzymes

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Correct Option: C
Explanation:
A zinc finger is a small protein structural motif that is characterized by the coordination of one or more zinc ions (Zn2+) in order to stabilize the fold. This proves that it does not require any DNA fingerprinting techniques.
So the correct option is 'Zinc finger analysis'.