Tag: enzymes classification and cofactors

Questions Related to enzymes classification and cofactors

Endopeptidase of plant kingdom is

  1. Trypsin

  2. Pepsin

  3. Papain

  4. Urease


Correct Option: C
Explanation:

Endopeptidase are the proteolytic enzymes which cleave the peptide chain at internal locations. They break peptide bonds of non terminal amino acids (i.e. within the molecule), in contrast to exopeptidases, which break peptide bonds from their end-pieces. One example is papain obtained from unripe papaya.

One of the following enzyme is used to cut genes at specific sites, therefore, useful in recombinant technology

  1. Ligase

  2. Topoisomerase

  3. Pepsin

  4. Restriction endonuclease


Correct Option: D
Explanation:

Restriction endonuclease are a part of cellular defense system in bacteria. These enzymes cleave or restrict external or exogenous DNA. A type of these enzymes act on specific palindromic sequences which they recognize.

An organic substance bound to an enzyme and essential for its activity is called as

  1. Apoenzyme

  2. lsoenzyme

  3. Coenzyme

  4. Holoenzyme


Correct Option: C
Explanation:

Enzymes are simple if they are made up of only proteins, e.g., pepsin, amylase, etc., while conjugate enzymes have an additional non-protein cofactor, e.g., dehydrogenase enzymes. The cofactor can be organic or inorganic. Loosely attached organic cofactor are called coenzymes, e.g., NAD, FAD etc., while firmly attached one is prosthetic group, e.g., haeme, biotin etc.