Tag: dna fingerprinting

Questions Related to dna fingerprinting

Microsatellite sequences are repeat units with base pairs

  1. 1 - 6

  2. 5 - 30

  3. 11 - 60

  4. 20 - 120


Correct Option: A
Explanation:

Microsatellites are polymorphic repeating units of 1–6 base pairs in length, found in human DNA. Microsatellites can be amplified for identification by PCR and can be used as molecular markers. Microsatellite analysis (MSA) is a PCR analysis of DNA in exfoliated urine cells.

So, the correct answer is '1-6.'

Hypervariable repeats/minisatellite DNA sequences were discovered by

  1. Jeffreys et al

  2. Sharp and Roberts

  3. Parsons et al

  4. Sears and Sears


Correct Option: A
Explanation:

Discovering their high level of variability, Sir Alec Jeffreys developed DNA fingerprinting based on minisatellites, solving the first immigration case by DNA in 1985, and the first forensic murder case, the Enderby murders in the United Kingdom, in 1986.

So, the correct answer is 'Jeffreys et al.'

Satellite DNA contains

  1. Repetitive DNA

  2. Segments forming sn RNA

  3. Segments forming sc RNA

  4. Both B and C.


Correct Option: A
Explanation:

Satellite DNA consists of arrays of tandemly repeating sequences that are widely present (>30%) in the genome. Satellite DNA is mainly present in heterochromatin or the tightly packed regions of chromosomes in centromeres, telomeres, and sometimes even in the euchromatin region (active region of the genome).

So, the correct answer is 'Repetitive DNA.'

Which is different in related species?

  1. Position of centromeres

  2. Position of NOR

  3. Satellite DNA

  4. All the above


Correct Option: C
Explanation:

Satellite DNA consists of arrays of tandemly repeating sequences that are widely present (>30%) in the genome. Satellite DNA is mainly present in heterochromatin or the tightly packed regions of chromosomes in centromeres, telomeres, and sometimes even in the euchromatin region (active region of the genome).

So, the correct answer is 'Satellite DNA.'

The technique used in DNA fingerprinting is 

  1. Western blotting

  2. Northern blotting

  3. Southern blotting

  4. Flow cytometry


Correct Option: C

VNTRs are

  1. Variable number of tandem repeats

  2. Very narrow tandem repeats

  3. Variable noncistronic transposon repeats

  4. Valuable noncistronic transposic regions


Correct Option: A

For DNA finger printing, DNA is obtained from

  1. White blood corpuscles

  2. Hair root cells

  3. Body secretions

  4. All the above


Correct Option: D

In DNA finger printing, the sequences which are matched are

  1. Minisatellite DNA

  2. Moderately repetitive sequences

  3. Microsatellite DNA

  4. Satellite DNA


Correct Option: A

DNA probes used in finger printing are

  1. Highly sensitive electron microscope

  2. UV beams

  3. DNA segments having radioactive isotopes

  4. X-ray scanners


Correct Option: C

The probe used initially by Alec Jaffreys during development of DNA finger printing was

  1. Ribozyme

  2. Sex chromosomes

  3. VNTR

  4. rDNASNP


Correct Option: D