Tag: instruments in biotechnology

Questions Related to instruments in biotechnology

DNA finger printing is useful for study of 

zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

  1. Forensics

  2. Polymorphism

  3. Identity and relationships

  4. All the above

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Correct Option: D

Basis of DNA finger printing is

zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

  1. Double helix

  2. Error in base sequence

  3. Polymorphism in sequence/satellite DNA

  4. DNA coiling

  5. DNA replication

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Correct Option: C

Which cannot be used for DNA finger-printing in humans ?

zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

  1. Leucocytes

  2. Erythrocytes/RBCs

  3. Hair bulbs

  4. Sperms

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Correct Option: B

In DNA finger printing, analysis is made of

zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

  1. Satellite DNA

  2. Moderately repetitive sequences

  3. Microsatellites

  4. Variable number of tandem repeats

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Correct Option: D

Process used for amplication or multiplication of DNA for finger printing is

zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

  1. Polymerase chain reaction

  2. Nesslerisation

  3. Southern blotting

  4. Northern blotting

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Correct Option: A

Enzyme that catalyses union of DNA fragments is

zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

  1. Ligase

  2. Polymerase

  3. Helicase

  4. Endonuclease

  5. Lipase

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Correct Option: A

DNA finger printing can resolve

zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

  1. Identification of a person

  2. Paternity dispute

  3. Maternity dispute

  4. All the above

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Correct Option: D

The probes for DNA finger printing are

zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

  1. Unknown single stranded labelled DNA

  2. Unknown double stranded labelled DNA

  3. Known single stranded labelled DNA

  4. Known double stranded unlabelled DNA

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Correct Option: C

Select the correct sequences of steps in DNA finger printing involving Southern blot hybridisation using radiolabelled VNTR as proble
I. Hybridisation using labelled VNTR prob
II. Isolation of DNA
III. Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon
IV. Detection of hybridised DNA fragments by autoradiography
V. Separation of DNA fragments by electropheresis
VI. Digestion of DNA by restriction endonucleases

zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

  1. I, V, VI, II, III and IV

  2. II, VI, V, III, I and IV

  3. V, I, VI, III, IV and II

  4. II, I, V, VI, IV and III

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Correct Option: B

Repeated genes are useful

zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

  1. Against mutations

  2. Formation of pseudogenes

  3. Formation of multiple copies of a product

  4. All of the above

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Correct Option: D
Explanation:

Repeated DNA sequences can act as more or less discrete elements in the genome. They can vary in the number of copies of repeat units from one chromosome to the next (polymorphism). The repeat copy number can expand through a unique form of mutation process referred to as ‘dynamic mutation.’ They are useful in the formation of pseudogenes and also in making multiple copies of a product.

So, the correct answer is 'All of the above.'