Tag: instruments in biotechnology

Questions Related to instruments in biotechnology

The technique of DNA finger printing relies on

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  1. Repetitive DNA

  2. Minisatellite DNA

  3. Both A and B

  4. None of the above

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Correct Option: C

Assertion : Thermographic imaging is a unique technique.

Reason : Thermographic imaging has some relation with DNA finger printing. 

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  1. If both assertion and reason are true and the reason is the correct explanation of the assertion.

  2. If both assertion and reason are true but reason is not the correct explanation of the assertion.

  3. If assertion is true but reason is false.

  4. If the assertion and reason both are false.

  5. If assertion is false but reason is true.

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Correct Option: B

For DNA fingerprinting to associate a blood sample with the criminal, what is minimally required

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  1. One sample of DNA, PCR amplification, restriction enzymes, and gel electrophoresis

  2. One sample of DNA, restriction enzymes, a DNA synthesizer, and gel electrophoresis

  3. Two samples of DNA, PCR amplification, restriction enzymes, and gel electrophoresis

  4. Two samples of DNA, PCR amplification, restriction enzymes, and a DNA synthesizer

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Correct Option: C
Explanation:

Two samples of DNA are required, one from the site of crime while another from the suspected criminal. These both samples of DNA are matched by DNA fingerprinting which involves identifying patterns of DNA known as a variable number of tandem repeats. PCR amplification is used to amplify DNA segments and restriction enzymes for cutting of DNA segments. Gel electrophoresis is used to separate these cut DNA segments based on their size. 

So, the correct option is 'Two samples of DNA, PCR amplification, restriction enzymes, and gel electrophoresis'.

Arrange the various steps of DNA fingerprinting technique in the correct order
(i) Separation of DNA fragments by electrophoresis
(ii) Digestion of DNA by restriction endonucleases
(iii) Hybridization using labeled VNTR probe
(iv) Isolation of DNA
(v) Detection of hybridized DNA fragments by autoradiography
(vi) Transferring the separated DNA fragments to nitrocellulose membrane

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  1. (ii) $\rightarrow$ (i) $\rightarrow$ (iv) $\rightarrow$ (vi) $\rightarrow$ (iii) $\rightarrow$ (v)

  2. (iv) $\rightarrow$ (i) $\rightarrow$ (ii) $\rightarrow$ (iii) $\rightarrow$ (vi) $\rightarrow$ (v)

  3. (iv) $\rightarrow$ (ii) $\rightarrow$ (i) $\rightarrow$ (vi) $\rightarrow$ (iii) $\rightarrow$ (v)

  4. (iii) $\rightarrow$ (v) $\rightarrow$ (iv) $\rightarrow$ (ii) $\rightarrow$ (i) $\rightarrow$ (vi)

Show answer
Correct Option: C
Explanation:
The technique of DNA Fingerprinting was initially developed by Alec Jeffreys. It included : -
  1.  isolation of DNA,
  2.  digestion of DNA by restriction endonucleases,
  3.  separation of DNA fragments by electrophoresis,
  4. transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon, 
  5. hybridisation using labelled VNTR probe, and
  6. detection of hybridised DNA fragments by autoradiography.
So, the correct option is '(iii) → (v) → (iv) → (ii) → (i) → (vi)'.