Tag: tissue culture

Questions Related to tissue culture

The technique of producing large number of genetically similar plants within short time by tissue culture is called as _____________.

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Organogenesis

  2. Somatic hybridization

  3. Micro-propagation

  4. Protoplast culture

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Correct Option: C
Explanation:

Micro-propagation means the propagation of plants by growing plantlets in tissue culture and then planting them out.

Rapidly dividing unorganised mass of cells in tissue culture is

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Callose.

  2. Callus.

  3. Embryoid.

  4. Plantlet.

Show answer
Correct Option: B
Explanation:

Tissue culture is a growth of cells or tissues separate from the organism in an artificial environment. The process is called as micropropagation in plant tissue culture. In tissue culture, the small piece of plant or animal is used to grow complete individual and those small parts are called as explants whereas complete grown individuals are called clones. The explant initially turns into rapidly dividing unorganised mass of cells on solid or liquid culture and that is called a callus. 

The following cells cannot be grown under tissue culture conditions 

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Hela cells

  2. Leucocytes

  3. Kidney cells

  4. Nerve cells

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Correct Option: D
Explanation:
Nerve cells cannot replicate under Tissue culture conditions hence they cannot be grown by the tissue culture technique. 
So the correct option is D.

Tissue culture was developed by

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Haberlandt

  2. Laibach

  3. White

  4. Steward

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Correct Option: C
Explanation:

In 1902 C.Haberlant was the first attempted to culture isolated plant cells invitro on artificial medium. But the discovery of the use of auxin is done by three scientists Gautherel, Nobecourt, and White. This discovery is the second phase of plant tissue culture. Then White discovery's proves that plant could be successfully regenerated from undifferentiated tissue or even single cell in culture called tissue culture. Then tissue culture was developed by P R White in 1925.
So the correct option is C.

Tissue used by Steward et. al. (1957) to prove cellular totipotency was

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Pith of root

  2. Pith of stem

  3. Phloem of root

  4. Phloem of stem

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Correct Option: C

What is "tissue culture"?

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Cultivation of tissue in laboratory through formation of new cells

  2. Introduction of new tissue in an animal body

  3. A technique for maintaining fragments of cells alive after their removal from an organism

  4. Maintaining tissue alive by immersing it partially in a nutrient fluid

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Correct Option: A
Explanation:
  • Tissue culture is the growth of tissues or cells separate from the organism. This is typically facilitated via the use of a liquid, semi-solid, or solid growth medium, such as broth or agar. 
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants.

First successful tissue culture was that of

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Tomato root

  2. Carrot root

  3. Potato stem

  4. Tobacco callus

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Correct Option: A

Cellular totipotency means

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Synthesis of new cells.

  2. Formation of new species.

  3. Formation of new plants.

  4. Capability of plant cell to form complete plant.

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Correct Option: D
Explanation:

Cellular totipotency means the ability of a cell to give rise to complete individual i.e., the formation of the complete cellular organisation from a single cell. Plant cells are totipotent as they can give rise to complete plant. So answer D is correct.

The structure employed by White for first successful tissue culture was

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Leaf cells

  2. Root of Carrot

  3. Root of Tomato

  4. Apical meristem

Show answer
Correct Option: C
Explanation:
White was the first one to successfully attempt the technique of tissue culture. He cultured tomato roots in a nutrient media composed of vitamins. He developed the continuously growing callus from the root which had the property of totipotency. The totipotent cells of the roots of tomato undergo cell division and form a mass of active cell known as callus. Callus when treated with auxin it promotes rooting and when it is treated with cytokinin it stimulates cell maturation, differentiation and finally, promotes the development of stem.
So, the correct answer is option C.

Tissue culture technique was first attempted by

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Haberlandt

  2. Hanning

  3. Nobecourt

  4. Gautheret

Show answer
Correct Option: A
Explanation:

Tissue culture is a biological technique which involves the development of plant under the controlled condition on a nutrient culture medium of the known composition. The pant developed by the tissue culture are cloned by the process of micropropagation. Gottlieb Haberlandt was the first to attempt tissue culture. He was the first to point out the possibilities of the culture of isolated tissues. He is known as the father of tissue culture.

So, the correct answer is option A.