Tag: electrophoresis

Questions Related to electrophoresis

The technique used for separating molecules by passing electric current is

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Fractionation

  2. Centrifugation

  3. Electrophoresis

  4. ELISA

  5. Chromatography

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Correct Option: C
Explanation:
Electrophoresis is a technique, in which particles of different sizes and charges are separated due to their movement to different distances under the influence of an electric field, e.g : nucleic acid, proteins, amino acids, nucleotides etc.
Chromatography is a technique of separating molecular components of a mixture by using the principle of partition coefficient.
Cell fractionation is the technique of separating and rupturing cells and separating the cellular components for study. It has two steps - homogenisation (rupturing of cells) and differential centrifugation (separation of different components of homogenate). 
ELISA stands for Enzyme Linked immunosorbent Assay. It is an assay procedure used in diagnosis of AIDS.

A method which uses paper or film with different electric poles at the two ends and is used for separation of charged particles is called

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Electrolysis

  2. Electrophoresis

  3. Electroplating

  4. Thin layer chromatography

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Correct Option: B

Agarose gel used to separate

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Carbohydrates.

  2. Fats.

  3. Proteins.

  4. Both A and B.

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Correct Option: C

Electric field is used in separating chemicals in which method?

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Ion exchange chromatography

  2. Electrophoresis

  3. Thin layer chromatography

  4. Column chromatography

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Correct Option: B

Electrophoresis is employed for

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Separating cell components.

  2. Separating charged particles.

  3. Reverse osmosis.

  4. In vitro storage of cell components.

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Correct Option: A

The base material that can be used in electrophoresis is

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Polyacrylamide.

  2. Agarose.

  3. Carrageenan.

  4. Both A and B.

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Correct Option: D

In DNA segment of six coils, 22 bp are linked by two hydrogen bonds. How many cytosine bases would be present

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. 22

  2. 38

  3. 44

  4. 76

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Correct Option: B

In a DNA fragment, there are 8 turns with 40% of the bases are cytosine. What would be the number of hydrogen bonds present in this DNA fragment 

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. 96

  2. 192

  3. 224

  4. 60

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Correct Option: C

Which one of the following statements is wrong with respect to separation of DNA fragments on gel electrophoresis

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. DNA fragments move towards anode under electric field through the matrix

  2. The commonly used matrix is agarose gel

  3. DNA fragments resolve according to their size

  4. The smaller DNA fragments separate first

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Correct Option: D

Escherichia coli, in which both the strands of DNA are labeled  with $^{15}N$ is transferred to $^{14}N$ medium and allowed to replicate for three generations. Find out the number of hybrid DNA molecules in the third generation

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. 8

  2. 2

  3. 12

  4. 10

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Correct Option: B