Tag: electrophoresis

Questions Related to electrophoresis

The first Nif genes were isolated from __________________.

botany biotechnology: principles and processes manipulation of dna electrophoresis isolation and separation of dna

  1. Klebsiella aerogenes

  2. Klebsiella oxytoa

  3. Klebsiella pneumonia

  4. Klebsiella granulonatis

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Correct Option: C

Insect-resistant transgenic cotton has been produced by inserting a piece of DNA from.

botany biotechnology: principles and processes manipulation of dna electrophoresis isolation and separation of dna

  1. An insect

  2. A bacterium

  3. A wild relative of cotton

  4. A virus

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Correct Option: A

One strand of the given DNA segment codes for mRNA having the sequence AUC GCG UCA for the synthesis of protein. Which DNA strand is responsible for the above mRNA sequence ________________.

botany biotechnology: principles and processes manipulation of dna electrophoresis isolation and separation of dna

  1. ATC-TAG

    GCC-GGC

    ATU-TAG

  2. TAG-ATC

    CGC-GCG

    AGT-TCA

  3. TGA-ACT

    CGC-GCG

    TAG-ATC

  4. AGA-TCT

    GCG-CGC

    GAT-CTA

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Correct Option: A

A technique utilized by a group of biologists to isolate an antifreeze gene found in cold water species of fish and introduce it into the genome of a different organism is called as 

botany biotechnology: principles and processes manipulation of dna electrophoresis isolation and separation of dna

  1. Cloning

  2. Artificial selection

  3. Selective breeding

  4. Genetic engineering

  5. Sexual recombination

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Correct Option: D
Explanation:
Genetic engineering is the branch of science which deals with the modification of genes. The desired genes is isolated from the host like the gene for antifreeze protein and are inserted into the vectors. The vectors carries the foreign gene into the desired organism like the fish and then the gene gets introduced into the genome of fish, resulting in the production of the protein.
Thus, the correct answer is option 'Genetic engineering'.

Having become an expert on gel electrophoresis, you are asked to examine a gel for a colleague. Where would you find the smallest segments of DNA?

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Near the positive electrode, farthest away from the wells

  2. Near the negative electrode, close to the wells

  3. Near the negative electrode, farthest away from the wells

  4. Near the middle, they tend to slow down after the first few minutes

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Correct Option: A
Explanation:

Since DNA is itself negatively charged, it would move towards the positive electrode. In gel electrophoresis, DNA fragments are separated on the basis of charge and masses. Thus, smaller the DNA fragment farther it moves from the well.

So, the correct option is A.

Gel electrophoresis is used for

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Construction of recombinant DNA by joining with cloning vectors

  2. Isolation of DNA molecules

  3. Cutting of DNA into fragments

  4. Separation of DNA fragments according to their size

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Correct Option: D
Explanation:

Gel electrophoresis is used to separate macromolecules like DNA, RNA and proteins. DNA fragments are separated according to their size. 

So, the correct option is 'Option D' .

Select the correct option to fill up the blanks.


(i) _____ is a natural polymer extracted from ______.

(ii) The DNA fragments purified by gel electrophoresis are used in constructing _____ by joining them with ______.
(iii) The ligation of alien DNA is carried out at a _____ present in one of the two ____ in a plasmid vector.
(iv) _____ enzyme remains active during the high temperature-induced denaturation of ds DNA. DNA fragments are resolved according to their ______
(v) through _____ in agarose gel electrophoresis.

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. (i) Agarose, seaweeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase, size(v) sieving effect.

  2. (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect

  3. (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii A-DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect

  4. (i) Size, sieving effect (ii) agarose, sea weeds (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes

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Correct Option: A
Explanation:

Agarose is a natural polymer extracted from sea weeds. The DNA fragments purified by gel electrophoresis are used in constructing recombinant DNA by joining them with the cloning vector. The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes in a plasmid vector. Taq polymerase enzyme remains active during the high temperature-induced denaturation of dsDNA.  DNA fragments are resolved according to their size through a sieving effect in agarose gel electrophoresis.

So, the correct option is 'Option A'.

Anticodon that aligns with initiator codon is

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. 5' - UAC - 3'

  2. 5 - UCA -3'

  3. 3' UAC - 5

  4. 3 CAU 5

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Correct Option: C

Which of the following does not produce sticky ends?

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Sma I

  2. EcoRI

  3. SalI

  4. BamHI

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Correct Option: A

Which one of the following methods requires two genetically variable individuals to produce the progeny?

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Cutting

  2. Layering

  3. Budding

  4. Goatee

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Correct Option: A