Tag: electrophoresis

Questions Related to electrophoresis

Fragments of DNA formed after treatment with endonucleases are separated by the technique

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Polymerase chain reaction

  2. Southern blotting

  3. Colony hybridisation

  4. Electrophoresis

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Correct Option: D

First step in Southern blot technique is

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Digestion of DNA by restriction enzyme

  2. Production of a group of genetically identical cells

  3. Denaturation of DNA on the gel for hybridisation with specific probe

  4. Denaturation of DNA from a nucleated cell as from the scene of crime

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Correct Option: A
Explanation:

The first step in a Southern blot technique is to prepare the DNA mixture by breaking it into smaller fragments using a restriction enzyme.

In gel electrophoresis, differential mobility of DNA depends upon

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Helical nature of DNA

  2. Double stranded nature of DNA

  3. Charge and size of DNA

  4. Hydrogen bonding between bases

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Correct Option: C

The term southern blotting refers to

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Transfer of DNA fragments from in vitro cellulose membrane to electrophoretic gel

  2. Attachment of probes to DNA fragments

  3. Transfer of DNA fragments from electrophoretic gel to nitrocellulose sheet

  4. Comparison of DNA fragments from two sources

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Correct Option: B

A correct pair of characteristics of molecular probe are:
a. Very long molecule
b. Double stranded
c. DNA or RNA
d. Complementary to a part of desired gene.

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. a,b

  2. b,c

  3. c,d

  4. d,a

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Correct Option: C

DNA fragments generated by restriction endonucleases in a chemical reaction can be separated by

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Restriction mapping

  2. Centrifugation

  3. Polymerase chain reaction

  4. Agarose gel electrophoresis

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Correct Option: D

When heated, the hydrogen bonds between the complementary strands of DNA break and the 2 strands separate in a process called melting. Which of the following pieces of DNA will require maximum temperature for melting?

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. $3'\quad AAGGTATACAAT\quad 5'\ 5'\quad TTCCATATGTTA\quad 3'$

  2. $3'\quad GAGCUAUCCGAG\quad 5'\ 5'\quad CUCGAUAGGCUC\quad 3'$

  3. $3'\quad ACGTCCGCTGCG\quad 5'\ 5'\quad TGCAGGCGACGC\quad 3'$

  4. $3'\quad ATTAGCTAGCAA\quad 5'\ 5'\quad TAATCGATCGTT\quad 3'$

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Correct Option: A

E.coli which was grown in the medium containing heavy isotope $ {  }^{ 15 }{ N }$ for several generations is shifted to normal $ _{  }^{ 14 }{ N }$ medium, the percentage of DNA strands with $ _{  }^{ 14 }{ N }$ isotope after two generations is _____________.

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. $12.5$%

  2. $50$%

  3. $75$%

  4. $100$%

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Correct Option: A

When a sample containing antibody $1\ gM$ is run on SDS - PAGE, what molecular weight bands are expected to be seen?

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. $70, 55. 15\ Kbp$

  2. $50, 15\ Kbp$

  3. $50, 55\ Kbp$

  4. $50, 25\ Kbp$

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Correct Option: A

Which of the following is not a technique for introducing recombinant DNA into host cells?

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Infection by Agrobacterium

  2. Microinjection

  3. Electrophoresis

  4. Infection by bacteriophage

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Correct Option: C
Explanation:

There are special techniques for the introduction of recombinant DNA into host cells. Microinjection, biolistics, infection by disarmed pathogens like Agrobacterium or bacteriophage are examples of such techniques. 

While electrophoresis is used for the separation of various DNA segments based on their length. 
So, the correct option is 'Electrophoresis'.