Tag: plant tissue culture

Questions Related to plant tissue culture

Plant piece used in tissue culture is called ____________.

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Explant

  2. Somaclone

  3. Inoculant

  4. Clone

Show answer
Correct Option: A

Axenic culture is

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Pure culture without any contamination

  2. Pure culture without any nutrient

  3. Culture of a tissue

  4. Culture of gene

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Correct Option: A

Callus is

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Plant hormone

  2. Root formation in culture media

  3. Plant by-product

  4. Undifferentiated mass of tissue

Show answer
Correct Option: D
Explanation:

Callus is a growing mass of unorganized plant parenchyma cells. It is mainly  used in plant tissue culture. So basically in other words, callus is a undifferentiated mass of tissue. 

Name the process by which thousands of plants  can be raised within limited time and space?

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Pathernocarpy

  2. Self pollination

  3. Tissue culture

  4. Water

Show answer
Correct Option: C
Explanation:

  • The process by which thousands of plants can be raised within limited time and space is Tissue culture.
  • When new plants are grown from a small piece of plant tissue or cells removed from the growing tips of a plant in a suitable growth medium is called tissue culture.
  • Its advantages are:-
1) Thousands of plantlets can be produced in a very less time from a small amount of plant tissue.
2) Very little space is needed for developing new plants.
3) The plants produced are disease free.

The best plant explants to raise virus free plant is?

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Embryo

  2. Vessels

  3. Meristem

  4. None

Show answer
Correct Option: C
Explanation:

Meristem is free from viruses and they are used to grow virus-free plant cultures. The meristem has the ability of rapid rate of mitotic cell division, which is much more than the viral rate of division. Hence, the virus is unable to spread through them, hence the meristems are almost always free of viruses.

So, the correct option is 'Meristem'.

Plant part used for tissue culture is sterilized by ____________.

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Chemicals

  2. Heat

  3. UV - rays

  4. Nutrient media

Show answer
Correct Option: A
Explanation:

Tissue culture is the growth of tissues or cells in an artificial medium separate from the organism. Plant parts can be sterilized by using several disinfecting agents i.e., use of chemicals.

Ex. :- Calcium hypo chloride, sodium hypo chloride, ethyl alcohol, silver nitrate etc.

In a sexual reproduction, two offspring's have the same genetic material and the same body features are called _______.

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Callus

  2. Twins

  3. Clones

  4. Mitosis

Show answer
Correct Option: B
Explanation:
  • In sexual reproduction, two offsprings have the same genetic material and the same body features are called identical twins.
  • They are two offspring produced by the same pregnancy.
  • Twins have the same genetic material and the same body features because they develop from a single fertilized egg, they have the same genome.
  • So, the correct answer is 'Twins'.

Which is used in tissue culture

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Explant

  2. Somaclones

  3. Hybridization

  4. None

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Correct Option: A

Tissue culture has been extensively used in research methods in all of the following fields of biology except in

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Bacteriology

  2. Embryology

  3. Virology

  4. None of the above

Show answer
Correct Option: D
Explanation:

Tissue culture is a collection of techniques used to grow cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition ( in vitro). The emergence of this technique became a turning point when polio virus, could be cultivated in vitro, a discovery of Enders, Weller, and Robbins (1949). This led to the feasibility of quantitative metabolic studies in animal viruses. Tissue culture is, therefore, today a vital part of life science research ( across plants and animals ) and in every area of research that includes bacteriology, embryology and virology.

Which increase shoot growth in callus culture?

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Cytokinin

  2. Auxin

  3. Gibberellin

  4. ABA

Show answer
Correct Option: A
Explanation:
  • The callus is an unspecialized, unorganized, growing and dividing the mass of cells.
  • It produced when explants are cultured on the appropriate solid medium, with both auxin and cytokinin in correct conditions.
  • In Callus culture, Auxins increase root growth and cytokinins increase shoot growth.
  • Hence Cytokinin increase shoot growth in callus culture.
  • So, the correct answer is 'Cytokinin'.