Tag: plant tissue culture

Questions Related to plant tissue culture

Plant medium used widely in preparation of culture medium is got from

  1. Cycas revoluta.

  2. Cocos nucifera.

  3. Pinus roxburghii.

  4. Borassus flabellifera.


Correct Option: B
Explanation:

Plant medium used widely in preparation of culture medium is obtained from the Cocos nucifera. It provides the growth hormone known as cytokinin. The first natural cytokinin known as zeatin was obtained from the coconut plant.

Cytokinins are the group of plant hormones which promotes cell division and differentiation in root and stem of the plant. It is involved in inducing morphogenesis. Auxin and cytokinin are used in equal quantity in the growth media of callus it promotes shoot and root formation and finally leads to organogenesis. 
So, the correct answer is option B.

Through which technique more female plants can be produced in papaya ___________.

  1. Genetic engineering

  2. Polyploid breeding

  3. Spraying ethephon

  4. Tissue culture


Correct Option: D

Callus is _____________.

  1. Tissue that forms embryo

  2. An insoluble carbohydrate

  3. Tissue that grows to from embryoid

  4. Unorganised actively dividing mass of cells maintained in culture


Correct Option: D

A totipotent cell means ________________.

  1. An undifferentiated cell capable of developing into a system or entire plant

  2. An undifferentiated cell capable of developing into an organ

  3. An undifferentiated cell capable of developing into complete embryo

  4. Cell which lacks the capability to differentiate into an organ or system.


Correct Option: A
Explanation:
A totipotent cell means an undifferentiated cell capable of developing into a system or entire plant.

The structure employed by White for first successful tissue culture was ____________.

  1. Root of Carrot

  2. Root of Tomato

  3. Leaf cells

  4. Apical meristem.


Correct Option: B
Explanation:

White was the first one to successfully attempt the technique of tissue culture. He cultured tomato roots in a nutrient media composed of vitamins. He developed the continuously growing callus from the root which had the property of totipotency.

Plant medium used widely in preparation of culture medium is got from ____________.

  1. Cycas revoluta

  2. Cocos nucifera

  3. Pinus roxburghii

  4. Borassus flabellifera


Correct Option: B
Explanation:

Option $B$ is correct.

Plant medium used widely in the preparation of culture medium is got from Cocos nucifera milk of coconut or coconut water is widely used for culture preparation as it contains several growth substances and can be extracted easily. It provides the growth hormone known as cytokinin. Cytokinins promote cell division and induce morphogenesis. The first natural cytokinin known as zeatin was obtained from the coconut plant.

Banana can be rapidly multiplied by ____________.

  1. Multiple shoot production

  2. Embryoid formation

  3. Shoot tip culture

  4. Callus formation


Correct Option: A

Who is known as father of plant tissue culture?

  1. Haberlandt

  2. Steward

  3. White

  4. Skoog.


Correct Option: A

Haploids are useful for ________________.

  1. Production of callus

  2. Production of homozygous diploids

  3. Microdensitometer studies

  4. Induction of giant cells


Correct Option: A

Which of the following statements are correct?
a. Callus is the disorganised mass of cells formed under in vitro condition.
b. Totipotency is the ability of a plant organ to regenerate an entire organism when cultured in a suitable medium.
c. Embryoid is the embryo-like structure formed under in vitro condition which has the potentiality to develop into a plantlet.
d. Explant is the excised portion of plant tissue or organ which is used in tissue culture study.
Select the correct answer using the codes given Codes.

  1. $1, 2$ and $4$

  2. $1, 3$ and $4$

  3. $1, 2$ and $3$

  4. $2, 3$ and $4$


Correct Option: A