Tag: cutting, insertion and ligation of dna

Questions Related to cutting, insertion and ligation of dna

Read the following statements carefully and select the correct option:
(A) Source of the restriction enzyme Hind III is E.coli
(B) In biolistic method of gene transfer, mircoparticles made up of gold or tungsten are coated with foreign DNA
(C) Micro-injection method for injecting recombinant DNA is used for animal cell
(D) Primers are chemically synthesized oligonucleotides that are complementary to the regions of DNA in PCR.
How many of the above statement are correct?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Four

  2. Three

  3. Two

  4. One

Show answer
Correct Option: B
Explanation:

A)

Restriction enzymes, also called restriction endonuclease, is a protein that cuts DNA after recognizing a specific sequence of DNA. Hind III is a type  II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA via hydrolysis.

B)

In the bolistic method of gene transfer, a gene gun is used to transfer exogenous or foreign DNA  in the cell. A droplet of tungsten powder and genetic material is a bullet. Gold is favored at the place of tungsten as it has better uniformity than tungsten and tungsten can be toxic to cells.

C) 
Micro-injection method for injecting recombinant DNA is used to produce transgenic mice. Harvested oocytes of the mouse are injected with the genetic material and then implanted in the oviduct of a pseudopregnant animal.

D)
A primer is a short strand of RNA or DNA that serves as a starting point for DNA synthesis.In vitro lab techniques that use DNA polymerase, DNA. primers are used. They are usually short, chemically synthesized oligonucleotides, with a length of about twenty bases.
Therefore A, B, and D are correct statements. Answer is B.

Use of transgenic plants as biological factories for the production of special chemicals is called as

biology option b: biotechnology and bioinformatics pcr cutting, insertion and ligation of dna gene cloning procedure

  1. Molecular farming

  2. Molecular genetics

  3. Molecular mapping

  4. Dry farming

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Correct Option: A
Explanation:
Molecular genetics is the study of structure and function of gene and regulation of its expression. It includes isolation and characterization of genes and identification of regulatory sequences. 
Molecular farming is transfer of genes of industrial importance to plants and animals for production of chemicals, for example production of transgenic animals for increased milk production. 
Molecular mapping is identification of loci of genes and their relative distance on chromosomes; DNA finger printing and genomic imprinting are most common techniques used for the purpose. 
Dry farming refers to crop production without irrigation, i.e., under limited moisture conditions; it is practiced for dry lands where water availability is low. 

Desired genes have been introduced into transgenic animals to obtain large-scale production of useful biological products encoded by these genes. This approach is generally referred to as _____________.

biology option b: biotechnology and bioinformatics pcr cutting, insertion and ligation of dna gene cloning procedure

  1. Hybridoma technology

  2. Molecular farming

  3. Gene therapyx

  4. Downstream processing

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Correct Option: B

A set of genes of cDNA is immobilized on a glass slide and used in transcriptome studies is called 

biology option b: biotechnology and bioinformatics pcr cutting, insertion and ligation of dna gene cloning procedure

  1. Proteome

  2. Microarray

  3. DNA chip

  4. Genome

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Correct Option: B
Explanation:

The transcriptome is the sum of all of its RNA transcripts. The information content of an organism is recorded in the DNA of its genome and expressed through transcription. A microarray is a laboratory tool used to detect the expression of thousands of genes at the same time. DNA microarray is microscopic glass slides that are printed with thousands of tiny spots in defined positions with each spot containing a known cDNA. The cDNA is made immobilized on a glass slide.

So, the correct answer is 'Microarray'.

Recently government of India has allowed mixing of alcohol in petrol. What is amount of alcohol permitted for mixing in petrol?

biology option b: biotechnology and bioinformatics pcr cutting, insertion and ligation of dna gene cloning procedure

  1. $25\%$

  2. $10-15\%$

  3. $10\%$

  4. $5\%$

Show answer
Correct Option: D
Explanation:

The amount is 5%.

Correct answer-D

A probe which is a molecule used to locate specific sequences in a mixture of DNA or RNA molecules could be __________________.

biology option b: biotechnology and bioinformatics pcr cutting, insertion and ligation of dna gene cloning procedure

  1. A single stranded RNA

  2. A single stranded DNA

  3. Either RNA or DNA

  4. Can be ssDNA but not ssRNA

Show answer
Correct Option: B
Explanation:

The molecular probes are usually single-stranded places of DNA (sometimes RNAs) labeled with radioisotopes such as phosphorus- 32. Molecular probes are available for many genetic disorders such as Duchenne muscular dystrophy, cystic fibrosis, Tay-Sachs disease.