Tag: cutting, insertion and ligation of dna

Questions Related to cutting, insertion and ligation of dna

Gene amplification using primers can be done by 

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Microinjection

  2. ELISA

  3. Polymerase chain reaction

  4. Gene gun

  5. Electrophoresis

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Correct Option: C

PCR proceeds in three distinct steps governed by temperature. The order of the steps is 

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Denaturation, Synthesis, Annealing

  2. Annealing, Synthesis, Denaturation

  3. Synthesis, Annealing, Denaturation

  4. Denaturation, Annealing, Synthesis

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Correct Option: D

What is true about DNA polymerase used in PCR?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. It is used to ligate introduced DNA in recipient cells.

  2. It serves as selectable marker.

  3. It is isolated from a virus.

  4. It is active at high temperature.

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Correct Option: D

Melting of DNA at 70$^o$ C is due to breakdown of

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Phosphodiester bonds

  2. Hydrogen bonds

  3. Glycosidic bonds

  4. Disulphide bonds

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Correct Option: B

Amplification of gene of interest by using PCR may go upto

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. 0.1 million

  2. 1.0 million

  3. 1.0 billion

  4. 1.0 trillion

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Correct Option: C

At which temperature a DNA molecule is denatured by heat in PCR

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. 70$^o$ -  80$^o$C

  2. 90$^o$ - 95$^o$C

  3. 42$^o$C

  4. 50$^o$ - 65$^o$C

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Correct Option: B

Taq polymerase is isolated from a

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Virus

  2. Bacterium

  3. Fungus

  4. Protozoan

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Correct Option: B

Taq polymerase is isolated from which bacteria?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. E. coli

  2. Bacillus thuringiensis

  3. Thermus aquaticus

  4. Agrobacterium

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Correct Option: C

Polymerase chain reaction (PCR) is used for

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. In vivo replication of specific DNA sequence using thermostable DNA polymerase

  2. In vitro synthesis of mRNAIn vivo synthesis of mRNA

  3. In vitro replication of specific DNA sequence using thermostable DNA polymerase

  4. Separation of DNA fragments according to their size

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Correct Option: C

Which one of the following is NOT a PCR-based molecular marker?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. RAPD

  2. AP-PCR

  3. DAF

  4. RELP

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Correct Option: A