Tag: cutting, insertion and ligation of dna

Questions Related to cutting, insertion and ligation of dna

Elution means

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Separation of DNA fragments on agarose gel

  2. Cutting and extraction of DNA bands from the agarose gel

  3. Making the DNA bands visible under UV radiation

  4. Isolation of alien DNA from the choice organism

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Correct Option: B

A dicotyledonous plant forms crown gall when

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Agrobacterium tumefaciens comes in contact with the plant

  2. Agrobacterium rhizogenes comes in contact with the plant

  3. A specific part of DNA from the Ti plasmid gets integrated with the plant chromosome

  4. A specific part of DNA from the Ri plasmid gets integrated with the plant chromosome

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Correct Option: C

Find the correct statement with respect to function or properties of RNA polymerase.

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Bacteria have $3$ different types of RNA polymerase

  2. RNA polymerase binds to promoter and initiate transcription

  3. It uses nucleoside monophosphate as substrate

  4. RNA polymerase polymerizes in a template independent manner

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Correct Option: A

Consider the following steps during recombinant DNA technology.
a. Breaking of donor DNA using restriction enzyme
b. Culture of cloned bacteria containing the fragment of donor DNA
c. Isolation of suitable plasmid
d. Insertion of recombinant DNA through cloning
e. Joining of donor DNA with a suitable plasmid using ligase
The correct sequence of these is?

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. $1, 3, 2, 4, 5$

  2. $3, 2, 1, 5, 4$

  3. $1, 3, 5, 4, 2$

  4. $5, 1, 3, 4, 2$

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Correct Option: A

When E coli with DNA $^{15}N ^{15}N $ is allowed to grow in a medium containing $^{14}N$ the number of DNA strands with $^{15}N$ after five generations is: 

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. 1

  2. 2

  3. 8

  4. 16

  5. 30

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Correct Option: A

Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Reverse transcriptase-production of.cDNA from mRNA

  2. DNA polymerase-used in polymerase chain reaction to amplify section of DNA

  3. DNA ligase-enzyme that cuts DNA, creating the sticky ends of restriction fragments

  4. Restriction enzyme-production of RFLPs

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Correct Option: C
Explanation:

Restriction enzymes cut DNA creating sticky ends of restriction fragments. While DNA ligase joins these sticky ends to form recombinant DNA. 

So, the correct option is 'DNA ligase-enzyme that cuts DNA, creating the sticky ends of restriction fragments'. 

After insertion of DNA segment within the sequence of Z-gene of bacteria, they are grown on chromogenic substrate. After the growth of bacterial colonies, they are identified as
i) Recombinants if colonies are blue- coloured.
ii) Recombinants if colonies are white coloured.
iii) Non-recombinants if colonies are blue- coloured.
iv) Non-recombinants if colonies are white coloured.

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. iii, iv

  2. i, iv

  3. i, ii

  4. ii, iii

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Correct Option: D

Enzyme' Taq polymerase' used in PCR, has been isolated from bacterium _________________.

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Agrobacterium tumefaciens

  2. Thermus aquaticus

  3. Streptomyces albus

  4. Escherichia coli

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Correct Option: B
Explanation:

The final step of PCR is the extension, wherein Taq DNA polymerase is isolated from a thermophilic bacterium Thermus aquaticus.

The polymerase chain reaction is a technique used for ____________.

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Amplification of DNA

  2. Amplification of enzymes

  3. Amplification of proteins

  4. All of these

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Correct Option: A
Explanation:

Polymerase chain reaction is a technique used for amplification of DNA fragments. Its amplification cycle involve three steps - denaturation, annealing and polymerisation which are repeated for 'n', cycles.

Which of the following statements are correct for the enzyme Taq polymerase?


(i) It remains active during the high temperature-induced denaturation of dsDNA.

(ii) It requires primers for carrying out the process of polymerisation.
(iii) It synthesizes the RNA region between the primers, using $dNTPs$ and $Mg^{2+}$ _______________.

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. (i) and (ii)

  2. (ii) and (iii)

  3. (i), (ii) and (iii)

  4. None of these

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Correct Option: A
Explanation:

The final step of PCR is the extension, wherein Taq DNA polymerase (isolated from a thermophilic bacterium Thermus aquaticus) synthesizes the DNA region between the primers, using dNTPs (deoxynucleoside triphosphates) and $Mg^{2+}$. The primers are extended towards each other so that the DNA segment lying between the two primers is copied. The optimum temperature for this polymerisation step is $72^o$$C$. Taq polymerase remains active during high temperature-induced denaturation of double-stranded DNA.