Tag: cutting, insertion and ligation of dna

Questions Related to cutting, insertion and ligation of dna

One of the major advantages of transforming bacteria with a plasmid that contains DNA of interest is to amplify the DNA.
What other laboratory technique can be used to achieve the same result?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Polymerase chain reaction

  2. Restriction fragment length polymorphism analysis

  3. DNA hybridization

  4. Gel electrophoresis

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Correct Option: A
Explanation:

Polymerase chain reaction is a method of amplifying a specific segment of DNA by making multiple copies of it, using primers and DNA polymerase

So, the correct answer is 'Polymerase chain reaction'

Who developed polymerase chain reaction technique and got Nobel Prize

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Muller

  2. Mullis

  3. Wilson

  4. Nirenberg

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Correct Option: B

Polymerase chain reaction is used for

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. In vivo replication

  2. In vivo synthesis of mRNA

  3. In vitro replication of DNA segment

  4. In vitro synthesis of mRNA

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Correct Option: C

PCR and RFLP are employed in

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. DNA sequencing

  2. Genetic engineering

  3. Study of enzymes

  4. Genetic transformation

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Correct Option: B

An analysis of chromosomal DNA using Southern hybridisation technique does not use

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Blotting

  2. Autoradiography

  3. PCR

  4. Electrophoresis

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Correct Option: C

Which ones are simple sequence repeats (SSRs)

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Minisatellite sequences

  2. Microsatellites

  3. Pseudogenes

  4. Multiple gene family.

Show answer
Correct Option: D
Explanation:

Simple sequence repeats (SSRs), sometimes described as genetic 'stutters,' are DNA tracts in which a short base-pair motif is repeated several to many times in tandem (e.g. CAGCAGCAG). These sequences experience frequent mutations that alter the number of repeats similar to Multigene Family, a set of genes descended by duplication and variation from some ancestral gene.

So, the correct answer is 'Multiple gene family.'

Polymerase chain reaction is useful in

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. DNA synthesis

  2. DNA amplification

  3. Protein synthesis

  4. Amino acid synthesis

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Correct Option: B

Polymerase chain reaction employs

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Primers and DNA ligase

  2. DNA ligase only

  3. DNA polymerase only

  4. Primers and DNA polymerase

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Correct Option: D

DNA polymerase or taq enzyme used in PCR is isolated from

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Thermus aquaticus

  2. E. coli

  3. Salmonella typhimurium

  4. None of these

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Correct Option: A
Explanation:

Taq polymerase is a thermostable DNA polymerase I obtained from the thermophilic bacterium Thermus aquaticus.


Source of Taq polymerase used in PCR is a

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Thermophilic fungus

  2. Mesophilic fungus

  3. Thermophilic bacterium

  4. Halophilic bacterium

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Correct Option: C
Explanation:

Taq polymerase is a thermostable DNA polymerase I obtained from the thermophilic bacterium Thermus aquaticus.