Tag: cutting, insertion and ligation of dna

Questions Related to cutting, insertion and ligation of dna

Which of the following can't be achieved using PCR ?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Detect HIV in AIDS suspect

  2. detect mutations in cancer patients

  3. Detect antigen - antibody interaction

  4. Detect specific micro organism from soil

Show answer
Correct Option: A

First technique and product produced by biotechnology are:

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. PCR and detection of disease

  2. ELISA and detection of AIDS

  3. PCR and medicines

  4. Fermentation and Ethanol

Show answer
Correct Option: A

Which of the following has popularised the PCR(Polymerase chain reactions)?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Easy availability of DNA template

  2. Availability of synthesis primers

  3. Availability of cheap deoxyribonucleotides

  4. Availability of thermostable DNA polymerase

Show answer
Correct Option: A

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. $5$' ______ GAATTC _____$3$

    $3$' _    ___ CTTAAG __________$5$'

  2. $5$' _______CACGTA ______ $3$'

    $3$'________ CTCAGT ________ $5$'

  3. $5$' ________ CGTTCG ________ $3$'

    $3$' ______ATGGTA ______$5$'

  4. $5$' _________ GATATG ______$3$'

    $3$'    __CTACTA _____$5$'

Show answer
Correct Option: A

The polymerase chain reaction is a technique used for?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Amplification of DNA

  2. Amplification of enzymes

  3. Amplification of proteins

  4. All of these

Show answer
Correct Option: A

Which of the following technique is employed to check the progression of a restriction enzyme digestion?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. PCR

  2. Bioreactor

  3. Gel electrophoresis

  4. Micro injection

Show answer
Correct Option: C
Explanation:

Answer is option C i.e. "Gel electrophoresis"
Gel electrophoresis is the technique which can check DNA fragment progression. Restriction enzyme digest DNA into fragments. Each fragment has different size. When gel electrophoresis is performed each fragment with different sizes are separated on the gel.
While, PCR is used for DNA amplification. Bioreactors are the fermentors used for fermentation process and micro-injection is a method used to transfer genes between animals.

Which of the following is not required in PCR?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. DNA primer

  2. DNA template

  3. RNA primer

  4. Taq polymerase

Show answer
Correct Option: A
Explanation:

DNA primer is not required for a PCR reaction. 

The reason for RNA primers to be used in PCR is the non availability of DNA primers. The RNA primers complimentary to the cellular DNA are easily synthesized by the DNA Primase enzyme which is nothing but RNA polymerase just like mRNA.

The areas of application of PCR include:


A. Production of monoclonal antibodies.
B. Insertion of recombinant DNA into an organism.
C. Diagnosis of a specific mutation.
D. Detection of plant pathogens.

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. A B

  2. B, C

  3. C, D

  4. A, B, C, D

Show answer
Correct Option: C
Explanation:

Answer is option C i.e. "C, D"

PCR is used for diagnosis of diseases in animals and plants, it is also used to identify mutations in DNA. Major applications of PCR are:
(1) Clinical Diagnosis
(2) DNA Sequencing
(3)  Gene Manipulation and Expression Studies
(4) Forensic Medicine and (5) Gene Cloning.

While, Production of monoclonal antibodies is done by Hybridoma technology and insertion of recombinant DNA into organisms is done by transformation or transduction.

Which of the following are all Nucleotides?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Adenosine, Cytidylic acid, Cytosine

  2. Adenylic acid, Cytidylic acid, Guanylic acid

  3. Cytidine, Adenine, Adenylic acid

  4. Uracil, Thymidine, Thymidylic acid

Show answer
Correct Option: B
Explanation:
  • Nucleotides are compound consisting of a nucleoside linked to a phosphate group. Nucleotides form the basic structural unit of nucleic acids such as DNA and RNA. For example, Adenylic acid, Guanylic acid, Thymidylic acid, and Cytidilic acid and 5'uridylic acid.
  • Hence Adenylic acid, Cytidilic acid, Guanylic acid are all Nucleotides.
  • So, the correct answer is 'Adenylic acid, Cytidilic acid, Guanylic acid'.

Kary B Mullis got Nobel prize for discovering 

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. DNA polymerase enzyme

  2. Polymerase chain reaction

  3. Restriction endonucleases

  4. Western blotting

Show answer
Correct Option: B
Explanation:

DNA polymerase enzyme synthesizes DNA strands by adding deoxyribonucleotides to 3' end of the primer via phosphodiester bonds; it uses parental DNA as a template. Kary Mullis got Nobel prize, in 1993 in chemistry, for the invention of polymerase chain reaction (PCR).  PCR - technique amplify the gene of interest.